Document
22-M09903 # 36 M2022-4843 JEM MOSCOW POLICE DEPARTMENT Page 1 of 27 Extraction Worksheets Differential DNA Extraction Form 202B-BI Batch Name: 073123SAW Work Station: DNA 3 Analyst: SAW Differential Extraction (F1) Date: 7/31/2023 4 ° C Start Time: 1500 150 µl PBS 091922 End Time: 0843 08/01/23 Date: 8/1/2023 Total samples: 14 Master Mix Made For: 16 ul / sample ul in Master Mix 56 ° C Heat Block: J1 SEB 020823 190 3040 Start Time: 0959 Prok 2449370 10 160 End Time: 1042 Exp. Date: 8/7/2024 Differential Extraction (F2) Manual 500 pl PBS 091922 x3 500 ul dH2O Sperm Lysis Total samples: 14 Master Mix Made For: 16 ul / sample ul in Master Mix SEB 020823 180 2880 56 ° C Heat Block: J1 DTT 042623 10 160 Start Time: 1242 Prok 2449370 10 160 End Time: 1408 Exp. Date: 8/7/2024 EZ1 Extraction EZ1 Kit Lot: 1750[REDACTED:US_SOCIAL_SECURITY_NUMBER]e: 8/1/2023 EZ1 Kit Exp: 12/18/2023 EZ1: F1-E EZ1 Protocol: Trace F2-F Elution Volume: 50ul Start time: F1-1406 F2-1419 Sample Name Sample Name 1 M2022-3494 Q3.1 13 RB1 073123SAW 2 M2023-2689 Q1.1 14 RB2 073123SAW 3 M2023-2287 Q1.2 15 4 M2023-2399 Q1.2 16 5 M2023-2399 Q1.4 17 6 M2023-2407 Q2.2 18 7 M2023-2407 Q2.5 19 8 M2023-2498 Q1.4 20 9 M2023-2532 Q1.2 21 10 M2023-2660 Q2.4 22 11 M2023-2513 Q1.7 23 12 M2023-2346 Q1.3 24 Note: F1 = Fraction 1, Non-sperm cell fraction F2 = Fraction 2, Sperm cell fraction Revision: 5 Issue Date: 12/27/2022 Extraction Worksheets Page: 1 of 4 Issuing Authority: Quality Manager 22-M09903 # 36 M2022-4843 JEM MOSCOW POLICE DEPARTMENT Page 2 of 27 Extraction Worksheets DNA Extraction Form 200-BI Batch Name: 073123SAW Work Station: DNA 2 Analyst: JEM Blood / Saliva / Tissue Total samples: 22 Master Mix Made For: 24 ul / sample ul in Master Mix Date: 7/31/2023 SEB 081122 490 11760 56 ° C Heat Block: G1 Prok 2449370 10 240 Start Time: 1531 7/31/23 Exp Date: 8/7/2024 End Time: 0929 8/1/23 N / A Hair Total samples: Master Mix Made For: ul / sample ul in Master Mix Date: SEB 0 56 ° C Heat Block: DTT 0 Start Time: Prok 0 End Time: Exp. Date: N / A Bone / Teeth Total samples: Master Mix Made For: ul / sample ul in Master Mix Date: SEB 0 56 ° C Heat Block: Prok 0 Start Time: Exp. Date: End Time: EZ1 Extraction Date: 8/1/2023 EZ1 Kit Lot: 1750107[REDACTED:US_SOCIAL_SECURITY_NUMBER] and F EZ1 Kit Exp: 12/18/2023 EZ1 Protocol: Large Volume Start time: 1125 Elution Volume: 50 ul Sample Name 27 0 54 0 1 M2023-2306 Q1.4 28 0 55 0 2 M2023-2310 Q1.6 29 0 56 0 3 M2023-2323 Q1 30 0 57 0 4 M2023-2323 Q2 31 0 58 0 5 M2023-2360 Q1 32 0 59 0 6 M2023-2354 Q1 33 0 60 0 7 M2023-2387 Q2.1 34 0 61 0 8 M2023-2580 Q1.5 35 0 62 0 9 M2023-2648 Q4 36 0 63 0 10 M1997-1552 Q10.1 37 0 64 0 11 M1997-1552 Q10.2 38 0 65 0 12 M1997-1552 Q10.3 39 0 66 0 13 M1997-1552 Q10.4 40 0 67 0 14 C2022-2295 Q2.2 41 0 68 0 15 M2022-4843 Q132.1 42 0 69 0 16 M2023-2648 Q6.1 43 0 70 0 17 M2023-2648 Q6.2 44 0 71 0 18 M2023-2513 Q1.6 45 0 72 0 19 M2023-2513 Q1.9 46 0 73 0 20 M2023-2648 Q5 47 0 74 0 21 LVRB1 073123JEM 48 0 75 0 22 LVRB2 073123JEM 49 0 76 0 23 0 50 0 77 0 24 0 51 0 78 0 25 0 52 0 79 0 26 0 53 0 80 0 Revision: 5 Issue Date: 12/27/2022 Extraction Worksheets Page: 2 of 4 Issuing Authority: Quality Manager 22-M09903 # 36 M2022-4843 JEM MOSCOW POLICE DEPARTMENT Page 3 of 27 Extraction Worksheets DNA Extraction Form 200-BI Batch Name: 073123SAW Work Station: DNA 2 Analyst: JEM Blood / Saliva / Tissue Total samples: 2 Master Mix Made For: 3 ul / sample ul in Master Mix Date: 7/31/2023 SEB 081122 490 1470 56 ° C Heat Block: G4 Prok 2449370 10 30 Start Time: 1545 7/31/23 Exp Date: 8/7/2024 End Time: 0955 8/1/23 N / A Hair Total samples: Master Mix Made For: ul / sample ul in Master Mix Date: SEB 0 56 ° C Heat Block: DTT 0 Start Time: Prok 0 End Time: Exp. Date: N / A Bone / Teeth Total samples: Master Mix Made For: ul / sample ul in Master Mix Date: SEB 0 56 ° C Heat Block: Prok 0 Start Time: Exp. Date: End Time: EZ1 Extraction Date: 8/1/2023 EZ1 Kit Lot: 175010707 [REDACTED:US_SOCIAL_SECURITY_NUMBER]1 Kit Exp: 12/18/2023 EZ1 Protocol: Large Volume Start time: 1125 Elution Volume: 50 ul Sample Name 27 0 54 0 1 M1997-1552 Q4.8.1 28 0 55 0 2 LVRB3 073123JEM 29 0 56 0 3 0 30 0 57 0 4 0 31 0 58 0 5 0 32 0 59 0 6 0 33 0 60 0 7 0 34 0 61 0 8 0 35 0 62 0 9 0 36 0 63 0 10 0 37 0 64 0 11 0 38 0 65 0 12 0 39 0 66 0 13 0 40 0 67 0 14 0 41 0 68 0 15 0 42 0 69 0 16 0 43 0 70 0 17 0 44 0 71 0 18 0 45 0 72 0 19 0 46 0 73 0 20 0 47 0 74 0 21 0 48 0 75 0 22 0 49 0 76 0 23 0 50 0 77 0 24 0 51 0 78 0 25 0 52 0 79 0 26 0 53 0 80 0 Revision: 5 Issue Date: 12/27/2022 Extraction Worksheets Page: 3 of 4 Issuing Authority: Quality Manager 22-M09903 # 36 M2022-4843 JEM MOSCOW POLICE DEPARTMENT Page 4 of 27 Extraction Worksheets DNA Extraction Form 200-BI Batch Name: 073123SAW Work Station: DNA 2 Analyst: JEM Blood / Saliva / Tissue Total samples: 2 Master Mix Made For: 3 ul / sample ul in Master Mix Date: 7/31/2023 SEB 081122 190 570 56 ° C Heat Block: F1 Prok 2449370 10 30 Start Time: 1547 7/31/23 Exp Date: 8/7/2024 End Time: 1005 8/1/23 N / A Hair Total samples: Master Mix Made For: ul / sample ul in Master Mix Date: SEB 0 56 ° C Heat Block: DTT 0 Start Time: Prok 0 End Time: Exp. Date: N / A Bone / Teeth Total samples: Master Mix Made For: ul / sample ul in Master Mix Date: SEB 0 56 ° C Heat Block: Prok 0 Start Time: Exp. Date: End Time: EZ1 Extraction Date: 8/1/2023 EZ1 Kit Lot: 175010707 EZ1[REDACTED:US_SOCIAL_SECURITY_NUMBER]it Exp: 12/18/2023 EZ1 Protocol: Trace TD Start time: 1130 Elution Volume: 200 ul Sample Name 27 0 54 0 1 M2023-3060 V1 28 0 55 0 2 KRB1 073123JEM 29 0 56 0 3 0 30 0 57 0 4 0 31 0 58 0 5 0 32 0 59 0 6 0 33 0 60 0 7 0 34 0 61 0 8 0 35 0 62 0 9 0 36 0 63 0 10 0 37 0 64 0 11 0 38 0 65 0 12 0 39 0 66 0 13 0 40 0 67 0 14 0 41 0 68 0 15 0 42 0 69 0 16 0 43 0 70 0 17 0 44 0 71 0 18 0 45 0 72 0 19 0 46 0 73 0 20 0 47 0 74 0 21 0 48 0 75 0 22 0 49 0 76 0 23 0 50 0 77 0 24 0 51 0 78 0 25 0 52 0 79 0 26 0 53 0 80 0 Revision: 5 Issue Date: 12/27/2022 Extraction Worksheets Page: 4 of 4 Issuing Authority: Quality Manager 22-M09903 # 36 M2022-4843 JEM MOSCOW POLICE DEPARTMENT Page 5 of 27 Extraction Worksheets DNA Extraction Form 200-BI Batch Name: 073123SAW Work Station: DNA 4 Analyst: KD Blood / Saliva / Tissue Total samples: 2 Master Mix Made For: N / A ul / sample ul in Master Mix Date: 8/1/2023 SEB 081122 490 N / A 56 ° C Heat Block: A1 Prok 2449370 10 N / A Start Time: 0725 Exp Date: 8/7/2024 End Time: 1230 N / A Hair Total samples: Master Mix Made For: ul / sample ul in Master Mix Date: SEB 0 56 ° C Heat Block: DTT 0 Start Time: Prok 0 End Time: Exp. Date: N / A Bone / Teeth Total samples: Master Mix Made For: ul / sample ul in Master Mix Date: SEB 0 56 ° C Heat Block: Prok 0 Start Time: Exp. Date: End Time: EZ1 Extraction Date: 8/1/2023 EZ1 Kit Lot: 175010707 EZ1: D[REDACTED:US_SOCIAL_SECURITY_NUMBER]Exp: 12/18/2023 EZ1 Protocol: Large Volume Start time: 1240 Elution Volume: 50ul (H20) Sample Name 27 0 54 0 1 M1999-1389 Q13.2 RE 28 0 55 0 2 LVRB1 080123KD 29 0 56 0 3 0 30 0 57 0 4 0 31 0 58 0 5 0 32 0 59 0 6 0 33 0 60 0 7 0 34 0 61 0 8 0 35 0 62 0 9 0 36 0 63 0 10 0 37 0 64 0 11 0 38 0 65 0 12 0 39 0 66 0 13 0 40 0 67 0 14 0 41 0 68 0 15 0 42 0 69 0 16 0 43 0 70 0 17 0 44 0 71 0 18 0 45 0 72 0 19 0 46 0 73 0 20 0 47 0 74 0 21 0 48 0 75 0 22 0 49 0 76 0 23 0 50 0 77 0 24 0 51 0 78 0 25 0 52 0 79 0 26 0 53 0 80 0 Revision: 5 Issue Date: 12/27/2022 Extraction Worksheets Page: 2 of 2 Issuing Authority: Quality Manager 22-M09903 # 36 M2022-4843 MOSCOW POLICE DEPARTMENT Page 6 of 27 JEM Fusion 6C DNA Worksheets DNA Quantitation Form 207A-BI 7500 Load Sheet Analyst: SAW Workstation: DNA 3 Repeat Pipettes Used: No Batch Name: 073123SAW Date: 8/1/2023 1 2 3 4 5 6 7 8 9 10 11 12 M2023- M2023- M2023- M2023- M2023- RB1_073123S M2023- A STD. 1A STD. 1B 2306_Q1.4 2648_Q4 2648_Q6.2 3060_V1 2399_Q1.4F2 AWF2 2407_Q2.5F1 M2023- M1997- M2023- KRB1_073123 M2023- RB2_073123S M2023- B STD. 2A STD. 2B 2310_Q1.6 1552_Q10.1 2513_Q1.6 JEM 2407_Q2.2F2 AWF2 2498_Q1.4F1 M1999- M2023- M1997- M2023- 1389_Q13.2_R M2023- M2022- M2023- C STD. 3A STD. 3B 2323_Q1 1552_Q10.2 2513_Q1.9 E 2407_Q2.5F2 3494_Q3.1F1 2532_Q1.2F1 M2023- M1997- M2023- LVRB1_08012 M2023- M2023- M2023- D STD. 4A STD. 4B 2323 Q2 1552 Q10.3 2648_Q5 3KD 2498_Q1.4F2 2689_Q1.1F1 2660_Q2.4F1 M2023- M1997- LVRB1_07312 M2022- M2023- M2023- M2023- E STD. 5A STD. 5B 2360_Q1 1552_Q10.4 3JEM 3494_Q3.1F2 2532_Q1.2F2 2287_Q1.2F1 2513_Q1.7F1 M2023- C2022- LVRB2_07312 M2023- M2023- M2023- M2023- F STD. 6A STD. 6B 2354 Q1 2295 Q2.2 3JEM 2689_Q1.1F2 2660_Q2.4F2 2399_Q1.2F1 2346_Q1.3F1 M2023- M2022- M1997- M2023- M2023- M2023- RB1_073123S G STD. 7A STD. 7B 2387 Q2.1 4843_Q132.1 1552_Q4.8.1 2287_Q1.2F2 2513_Q1.7F2 2399_Q1.4F1 AWF1 M2023- M2023- LVRB3_07312 M2023- M2023- M2023- RB2_073123S H NTCA NTCB 2580 Q1.5 2648 Q6.1 3JEM 2399_Q1.2F2 2346_Q1.3F2 2407_Q2.2F1 AWF1 Plexor HY Kit Lot #: 0000546224 Std. Prep. Date: 08/01/23 by SAW ΤΕ 081122 Exp. Date: 7/8/2024 7500: B Total Samples: 72 Master Mix: 800 ul Master Mix made for: 80 Primer / IPC Mix: 80 ul Water: 560 ul 18 uL Master Mix / well Issue Date: 07/14/2023 Revision: 2 Fusion 6C DNA Worksheets Page: 1 of 10 Issuing Authority: Quality Manager 22-M09903 # 36 M2022-4843 MOSCOW POLICE DEPARTMENT Page 7 of 27 JEM Fusion 6C DNA Worksheets Batch Name: 073123SAW Date: 8/1/2023 Analyst: SAW Autosomal Standard Curve 0731235AW.aan-Not for Medical Diagnostic Use. PCR Curves Sample IDs Standard Curves Reports FLR-Autosomal C0560-Y Curve 1 (group 1) Curve 1 (group 1) A1 Well STD Sample. 1A 10 a 17.4 Te 80.1 Conc 5.0E01 Yes Ts? St- y = -3.56x + 23.16 81 C1 STD STD.. 2A 3A 19.5 22.1 80.3 80.4 1.0E01 2.0E00 Yes Yes R = 0.998 E1 D1 STD STD.. 4A SA 24.3 27.1 80.6 80.5 8.0E 4.0E - - 02 01 Yes Yes Eff = 90.84% F1 STD. 6A 29.4 80.4 1.6E-02 Yes A2 G1 STD STD.. 18 7A 31.8 17.2 80.3 80.2 3.2E 5.0E01-03 Yes Yes 82 C2 STD STD.. 20 38 22.1 19.4 80.4 80.5 1.0E01 2.0E00 Yes Yes 8- D2 STD. 48 24.4 80.7 4.0E-01 Yes E2 F2 STD STD.. 58 68 30.1 27.1 80.7 80.5 8.0E 1.6E - - 02 02 Yes Yes 02 STD. 78 32.2 80.3 3.2E-03 Yes 我 一 8- ) Threshold 20- Cycle (Ct 2- 21 8- 2- 0.01 0.1 Concentration Revision: 2 Issue Date: 07/14/2023 Fusion 6C DNA Worksheets Page: 2 of 10 Issuing Authority: Quality Manager 22-M09903 # 36 M2022-4843 MOSCOW POLICE DEPARTMENT Page 8 of 27 JEM Fusion 6C DNA Worksheets Batch Name: 073123SAW Date: 8/1/2023 Analyst: SAW Y Standard Curve 073123SAW.aan-Net for Medical Diagnostic Use. PCR Curves Sample IDs Standard Curves | Reports FLR Curve-Autosomal 1 (group 2) C0560-Y Curve 1 (group 21 Well STD Sample. 1A ID 18.5 a T 81.4 Conc 5.0E01 Yes Tm? おー y = -3.50x + 24.25 81 C1 STO STD.. 2A ЗА 20.6 23.3 81.5 81.7 10E01 2.0E00 Yes Yes R = 0.999 Et 01 STD STD.. 4A SA 25.7 28.1 81.8 81.8 8.0E 4.0E - - 02 01 Yes Yes Eff = 93.00% F1 STD. 6A 30.6 81.8 1.6E-02 Yes A2 01 STD STD. 18 7A 18.4 33.3 81.4 81.7 3.2E 5.0E01-03 Yes Yes B2 C2 STD STD.. 28 38 20.7 231 81.6 81.8 1.0E01 2.0E00 Yes Yes E2 D2 STD STD.. 58 48 40 25.5 28.0 81.9 81.9 8.0E 4.0E - - 01 02 Yes Yes 02 F2 STD STD. 78 68 30.4 32.8 81.8 81.9 1.6E 3.2E - - 03 02 Yes Yes 8- 8- ম- ) Threshold 另一 Cycle (CE スー ম- - スー 8- 2- 0.01 0.1 Concentration Revision: 2 Issue Date: 07/14/2023 Fusion 6C DNA Worksheets Page: 3 of 10 Issuing Authority: Quality Manager 22-M09903 # 36 M2022-4843 MOSCOW POLICE DEPARTMENT Page 9 of 27 JEM Fusion 6C DNA Worksheets DNA Quantitation Form 207B-BI 7500 Results Sheet Batch Name: 073123SAW Analyst: SAW Normalize to Auto / Y: Auto Date: 8/2/2023 ΤΕ 081122 Workstation: DNA 2 Quantity Quantity ul Sample ng / µl ng / µl Auto / Y IPC for ul TE to ul to be Well Sample Name [Auto] [Y] Ratio Status Dilution be added ng / ul Final Amplified A3 M2023-2306 Q1.4 0.009 0.002 3.60 OK 0 0.0 0.0280 15.0 B3 M2023-2310 Q1.6 0.013 0.009 1.40 OK 0 0.0 0.0403 15.0 C3 M2023-2323 Q1 0.486 0.178 2.70 OK 5 19.3 0.1 15.0 D3 M2023-2323 Q2 0.511 0.243 2.10 OK 5 20.6 0.1 15.0 E3 M2023-2360 Q1 0.368 0.163 2.30 OK 5 13.4 0.1 15.0 F3 M2023-2354 Q1 1.980 1.440 1.40 OK 5 94.0 0.1 15.0 G3 M2023-2387 Q2.1 0.00013 N / A N / A OK 0 0.0 0.00041 15.0 H3 M2023-2580 Q1.5 0.019 0.003 6.90 OK 0 0.0 0.0621 15.0 A4 M2023-2648 Q4 0.025 0.018 1.40 OK 0 0.0 0.0790 15.0 B4 M1997-1552 Q10.1 0.031 0.003 11.60 OK 0 0.0 0.0998 15.0 C4 M1997-1552 Q10.2 0.101 0.016 6.20 OK 0 0.0 0.101 15.0 D4 M1997-1552 Q10.3 0.225 0.004 52.50 OK 10 12.5 0.1 15.0 E4 M1997-1552 Q10.4 0.105 0.007 14.50 OK 0 0.0 0.105 15.0 F4 C2022-2295 Q2.2 0.208 0.136 1.50 OK 10 10.8 0.1 15.0 G4 M2022-4843 Q132.1 1.060 N / A N / A OK 5 48.0 0.1 15.0 H4 M2023-2648 Q6.1 1.160 0.714 1.60 OK 5 53.0 0.1 15.0 A5 M2023-2648 Q6.2 1.130 0.824 1.40 OK 5 51.5 0.1 15.0 B5 M2023-2513 Q1.6 0.005 0.001 4.50 OK 0 0.0 0.0172 15.0 C5 M2023-2513 Q1.9 0.007 0.001 11.00 OK 0 0.0 0.0239 15.0 D5 M2023-2648 Q5 1.270 1.230 1.00 OK 5 58.5 0.1 15.0 E5 LVRB1 073123JEM N / A N / A N / A OK 0 0.0 0 15.0 F5 LVRB2 073123JEM N / A N / A N / A OK N / A N / A N / A N / A G5 M1997-1552 Q4.8.1 1.920 0.004 489.20 OK 5 91.0 0.1 15.0 H5 LVRB3 073123JEM N / A N / A N / A OK 0 0.0 0 15.0 A6 M2023-3060 V1 5.880 3.210 1.80 OK 5 289.0 0.1 15.0 B6 KRB1 073123JEM N / A N / A N / A OK 0 0.0 0 15.0 C6 M1999-1389 Q13.2 RE 0.043 0.023 1.8 OK 0 0.0 0.111 * 15.0 D6 LVRB1 080123KD N / A N / A N / A OK 0 0.0 0 * 15.0 E6 M2022-3494 Q3.1F2 0.955 1.260 0.80 OK 5 42.8 0.1 15.0 F6 M2023-2689 Q1.1F2 0.0041 0.003 1.40 OK 0 0.0 0.0132 15.0 G6 M2023-2287 Q1.2F2 3.090 3.870 0.80 OK 5 149.5 0.1 15.0 H6 M2023-2399 Q1.2F2 0.009 N / A N / A OK N / A N / A N / A N / A A7 M2023-2399 Q1.4F2 0.010 0.0004 25.50 OK 0 0.0 0.0317 15.0 B7 M2023-2407 Q2.2F2 0.079 N / A N / A OK N / A N / A N / A N / A C7 M2023-2407 Q2.5F2 0.0003 N / A N / A OK N / A N / A N / A N / A D7 M2023-2498 Q1.4F2 0.0074 N / A N / A OK N / A N / A N / A N / A E7 M2023-2532 Q1.2F2 0.0005 N / A N / A OK N / A N / A N / A N / A F7 M2023-2660 Q2.4F2 N / A N / A N / A OK N / A N / A N / A N / A G7 M2023-2513 Q1.7F2 N / A N / A N / A OK N / A N / A N / A N / A H7 M2023-2346 Q1.3F2 0.0004 N / A N / A OK N / A N / A N / A N / A A8 RB1 073123SAWF2 N / A N / A N / A OK 0 0.0 0 15.0 Revision: 2 Issue Date: 07/14/2023 Fusion 6C DNA Worksheets Page: 4 of 10 Issuing Authority: Quality Manager 22-M09903 # 36 M2022-4843 MOSCOW POLICE DEPARTMENT Page 10 of 27 JEM Fusion 6C DNA Worksheets DNA Quantitation Form 207B-BI 7500 Results Sheet Batch Name: 073123SAW Analyst: SAW Normalize to Auto / Y: Auto Date: 8/2/2023 ΤΕ 081122 Workstation: DNA 2 Quantity Quantity ul Sample ng / µl ng / µl Auto / Y IPC for ul TE to ul to be Well Sample Name [Auto] [Y] Ratio Status Dilution be added ng / ul Final Amplified B8 RB2 073123SAWF2 N / A N / A N / A OK N / A N / A N / A N / A C8 M2022-3494 Q3.1F1 0.186 0.210 0.90 OK 10 8.6 0.1 15.0 D8 M2023-2689 Q1.1F1 3.870 2.650 1.50 OK 5 188.5 0.1 15.0 E8 M2023-2287 Q1.2F1 0.552 0.009 61.40 OK 5 22.6 0.1 15.0 F8 M2023-2399 Q1.2F1 2.440 N / A N / A OK N / A N / A N / A N / A G8 M2023-2399 Q1.4F1 12.200 0.001 21514.40 OK 5 605.0 0.1 15.0 H8 M2023-2407 Q2.2F1 52.500 0.001 36363.90 OK N / A N / A N / A N / A A9 M2023-2407 Q2.5F1 0.248 0.031 7.90 OK 10 14.8 0.1 15.0 B9 M2023-2498 Q1.4F1 14.500 N / A N / A OK N / A N / A N / A N / A C9 M2023-2532 Q1.2F1 0.831 0.003 282.90 OK N / A N / A N / A N / A D9 M2023-2660 Q2.4F1 0.164 0.040 4.10 OK 10 6.4 0.1 15.0 E9 M2023-2513 Q1.7F1 0.034 0.001 50.30 OK 0 0.0 0.109 13.7 F9 M2023-2346 Q1.3F1 0.035 0.0001 271.00 OK N / A N / A N / A N / A G9 RB1 073123SAWF1 N / A N / A N / A OK 0 0.0 0 15.0 H9 RB2 073123SAWF1 N / A N / A N / A OK N / A N / A N / A N / A Note: For any concentrated samples, " ng / ul Final " reflects the new estimated concentration. Concentrate to 15ul Concentrate to 25ul Not Amplified Normalized by EMS on 08/02/2023 M1999-1389_Q13.2_RE was combined with M1999-1989_Q13.2 prior to concentration. LVRB1_080123KD was combined with LVRB_070723KNB prior to concentration. *: total ng / ul based on combined quant values. Revision: 2 Issue Date: 07/14/2023 Fusion 6C DNA Worksheets Page: 5 of 10 Issuing Authority: Quality Manager 22-M09903 # 36 M2022-4843 MOSCOW POLICE DEPARTMENT Page 11 of 27 JEM Fusion 6C DNA Worksheets STR Amplification Set-Up DNA Form 207C-BI Batch Name: 073123SAW Analyst: EMS Plate Name: 073123SAW Workstation: DNA 4 Repeat Pipettes Used: No Thermal Cycler A Date: 8/2/2023 1 2 3 4 5 6 7 8 9 10 11 12 M2023- LVRB3 07312 RB1 073123S A 2580_Q1.5 3JEM AWF1 M1999- M2023- M2023- M2023- 1389_Q13.2_R M2022- M2023- B 2306 Q1.4 2648_Q4 2648 _Q6.1 E 3494_Q3.1F1 3060 V1 M2023- M1997- M2023- LVRB1_08012 M2023- KRB1_073123 C 2310_Q1.6 1552_Q10.1 2648_Q6.2 3KD 2689_Q1.1F1 JEM M2023- M1997- M2023- M2022- M2023- D 2323_Q1 1552_Q10.2 2513 Q1.6 3494 Q3.1F2 2287 Q1.2F1 POS_CTRL M2023- M1997- M2023- M2023- M2023- E 2323_Q2 1552_Q10.3 2513_Q1.9 2689_Q1.1F2 2399_Q1.4F1 NEG_CTRL M2023- M1997- M2023- M2023- M2023- F 2360 Q1 1552 Q10.4 2648 Q5 2287 Q1.2F2 2407 Q2.5F1 M2023- C2022- LVRB1_07312 M2023- M2023- G 2354_Q1 2295_Q2.2 3JEM 2399_Q1.4F2 2660_Q2.4F1 M2023- M2022- M1997- RB1_073123S M2023- H 2387 Q2.1 4843 Q132.1 1552_Q4.8.1 AWF2 2513_Q1.7F1 PowerPlex Fusion 6C Kit Lot #: 0000543137 Total Samples: 42 Master Mix Made For: 46 Exp. Date: 12/1/2023 Reagent µl / sample µl in Master Mix 5x Master Mix 5 230 TE N / A Primers 5 230 H20 0 0 Master Mix / Sample 10 DNA Template 15 Total Rxn Volume 25 Issue Date: 07/14/2023 Revision: 2 Fusion 6C DNA Worksheets Page: 6 of 10 Issuing Authority: Quality Manager 22-M09903 # 36 M2022-4843 MOSCOW POLICE DEPARTMENT Page 12 of 27 JEM Fusion 6C DNA Worksheets 3500 Load Sheet Form 207D-BI DNA Analyst: EMS Batch Name: 073123SAW Date: 8/2/2023 Plate Name: 073123SAW Data Evaluation Analyst: MTK 1 2 3 4 5 6 7 8 9 10 11 12 M2023- LVRB3_07312 RB1_073123S A LADDER A01 2580 Q1.5 LADDER_A03 3JEM LADDER_05 AWF1 M1999- M2023- M2023- M2023- 1389 Q13.2 R M2022- M2023- B 2306 Q1.4 2648 Q4 2648 Q6.1 E 3494 Q3.1F1 3060 V1 M2023- M1997- M2023- LVRB1_08012 M2023- KRB1_073123 C 2310 Q1.6 1552_Q10.1 2648_Q6.2 3KD 2689_Q1.1F1 JEM M2023- M1997- M2023- M2022- M2023- D 2323 Q1 1552_Q10.2 2513_Q1.6 3494 Q3.1F2 2287_Q1.2F1 POS CTRL M2023- M1997- M2023- M2023- M2023- E 2323 Q2 1552_Q10.3 2513_Q1.9 2689 Q1.1F2 2399_Q1.4F1 NEG_CTRL M2023- M1997- M2023- M2023- M2023- F 2360 Q1 1552_Q10.4 2648 Q5 2287 Q1.2F2 2407_Q2.5F1 LADDER_F06 M2023- C2022- LVRB1_07312 M2023- M2023- G 2354 Q1 2295_Q2.2 3JEM 2399 Q1.4F2 2660 Q2.4F1 LADDER_G06 M2023- M2022- M1997- RB1_073123S M2023- H 2387 Q2.1 4843 Q132.1 1552 Q4.8.1 AWF2 2513 Q1.7F1 LADDER H06 COMMENTS: M2023-2306_Q1.4 reinjected due to ils failure Ladder in well A5 should be LADDER_A05 EMS 08/09/2023 Total Samples: 48 Master Mix made for: 54 LADDER_F06 failed and was not used for data analysis EMS 08/09/2023 HiDi Formamide 513 UL 3500: Pete Internal Lane Standard 27 uL 3500 POP4 Lot #: 2303219 HiDi Formamide Lot #: 2203820 Stabilizer Reagent N / A Exp. Date: 11/11/2023 Exp. Date: 4/21/2024 Master Mix / well 10 UL Stabilizer Reagent Lot #: N / A Anode Buffer Lot #: 2301827 Exp. Date: N / A Exp. Date: 11/4/2023 Cathode Buffer Lot #: 2301876 Exp. Date: 10/27/2023 Issue Date: 07/14/2023 Revision: 2 Fusion 6C DNA Worksheets Page: 7 of 10 Issuing Authority: Quality Manager 22-M09903 # 36 M2022-4843 MOSCOW POLICE DEPARTMENT Page 13 of 27 JEM Fusion 6C DNA Worksheets DNA Quantitation Form 207B-BI 7500 Results Sheet Batch Name: 073123SAW Analyst: EMS Normalize to Auto / Y: Auto Date: 8/4/2023 ΤΕ 081122 Workstation: DNA 3 Quantity Quantity ul Sample ng / µl ng / µl Auto / Y IPC for ul TE to ul to be Well Sample Name [Auto] [Y] Ratio Status Dilution be added ng / ul Final Amplified C4 M1997-1552 Q10.2 0.101 0.016 6.20 OK 0 0.0 0.222 15.0 D4 M1997-1552 Q10.3 0.225 0.004 52.50 OK 0 0.0 0.225 15.0 E4 M1997-1552 Q10.4 0.105 0.007 14.50 OK 0 0.0 0.231 15.0 Note: For any concentrated samples, " ng / ul Final " reflects the new estimated concentration. Concentrate to 15ul Revision: