DNA Extraction Worksheets 124

22-M09903 # 34 M2022-4843

MOSCOW POLICE DEPARTMENT Page 1 of 46 JEM Extraction Worksheets Differential DNA Extraction Form 202B-BI
Batch Name: 032023SAW Work Station: DNA 2 Analyst: JEM Differential Extraction (F1)
Date: 3/20/2023 4 ° C Start Time: 3/20/2023 1510 150 µl PBS 070722 End Time: 3/21/2023 0720
Date: 3/21/2023
Total samples: 14 Master Mix Made For: 16 ul / sample ul in Master Mix 56 ° C Heat Block: G1
SEB 020823 190 3040 Start Time: 0920
Prok 2344719 10 160 End Time: 1020
Exp. Date: 4/28/2023 Differential Extraction (F2) Manual 500 pl PBS 070722 x 3 500 ul dH2O Sperm Lysis
Total samples: 14 Master Mix Made For: 16 ul / sample ul in Master Mix SEB 020823 180 2880 56 ° C Heat Block: G1
DTT 083121 10 160 Start Time: 1303
Prok 2344719 10 160 End Time: 1408
Exp. Date: 4/28/2023 EZ1 Extraction
EZ1 Kit Lot: 1720[REDACTED:US_SOCIAL_SECURITY_NUMBER]e: 3/21/2023
EZ1 Kit Exp: 9/8/2023 EZ1: F1 = E
EZ1 Protocol: Trace F2 = F
Elution Volume: 50 ul Start time: F1 = 1406
F2 = 1422

Sample Name Sample Name
1 M2022-4967 Q1.3 13 RB1 032023JEM

2 P2022-3611 Q1.2 14 RB2 032023JEM

3 P2022-3611 Q1.5 15

4 M2022-5283 Q1.2 16

5 M2022-5062 Q2.2 17

6 M2022-5062 Q2.6 18

7 M2022-5079 Q1.4 19

8 M2022-5118 Q1.2 20

9 M2022-5118 Q1.5 21

10 M2022-4999 Q1.2 22

11 M2022-4999 Q1.5 23

12 M2022-4359 Q5.2 24

Note: F1 = Fraction 1, Non-sperm cell fraction F2 = Fraction 2, Sperm cell fraction
Revision: 5
Issue Date: 12/27/2022
Extraction Worksheets Page: 1 of 4 Issuing Authority: Quality Manager

22-M09903 # 34 M2022-4843

MOSCOW POLICE DEPARTMENT Page 2 of 46 JEM Extraction Worksheets Differential DNA Extraction Form 202B-BI
Batch Name: 032023SAW Work Station: DNA 2 Analyst: JEM Differential Extraction (F1)
Date: 3/20/2023 4 ° C Start Time: 3/20/2023 1515 150 µl PBS 070722 End Time: 3/21/2023 0720
Date: 3/21/2023
Total samples: 4 Master Mix Made For: 5 ul / sample ul in Master Mix 56 ° C Heat Block: G2
SEB 020823 190 950 Start Time: 0924
Prok 2344719 10 50 End Time: 1024
Exp. Date: 4/28/2023 Differential Extraction (F2) Manual 500 pl PBS 070722 x 3 500 ul dH2O Sperm Lysis
Total samples: 4 Master Mix Made For: 5 ul / sample ul in Master Mix SEB 020823 180 900 56 ° C Heat Block: G2
DTT 083121 10 50 Start Time: 1306
Prok 2344719 10 50 End Time: 1410
Exp. Date: 4/28/2023 EZ1 Extraction
EZ1 Kit Lot: 17203966[REDACTED:US_SOCIAL_SECURITY_NUMBER]/21/2023
EZ1 Kit Exp: 9/8/2023 EZ1: F1 / F2 = G
EZ1 Protocol: Trace
Elution Volume: 50 ul Start time: F1 / F2 = 1433 Sample Name Sample Name
1 M2023-1081 Q1.2 13

2 M2023-1081 Q1.3 14

3 RB3 032023JEM 15

4 RB4 032023JEM 16

5 17 6 18 7 19 8 20 9 21 10 22 11 23 12 24
Note: F1 = Fraction 1, Non-sperm cell fraction F2 = Fraction 2, Sperm cell fraction
Revision: 5
Issue Date: 12/27/2022
Extraction Worksheets Page: 2 of 4 Issuing Authority: Quality Manager

22-M09903 # 34 M2022-4843

MOSCOW POLICE DEPARTMENT Page 3 of 46 JEM Extraction Worksheets DNA Extraction Form 200-BI
Batch Name: 032023SAW Work Station: DNA 3 Analyst: SAW Blood / Saliva / Tissue
Total samples: 23 Master Mix Made For: 25 ul / sample ul in Master Mix Date: 3/21/2023 SEB 081122 490 12250 56 ° C Heat Block: J1
Prok 2344719 10 250 Start Time: 0949
Exp Date: 4/28/2023 End Time: 1106 N / A Hair
Total samples: Master Mix Made For:
ul / sample ul in Master Mix Date:
SEB 0 56 ° C Heat Block:
DTT 0 Start Time:
Prok 0 End Time:
Exp. Date:
N / A Bone / Teeth
Total samples: Master Mix Made For:
ul / sample ul in Master Mix Date:
SEB 0 56 ° C Heat Block:
Prok 0 Start Time:
Exp. Date: End Time:
EZ1 Extraction Date: 3/21/2023
EZ1 Kit Lot: 172039661 E[REDACTED:US_SOCIAL_SECURITY_NUMBER]
EZ1 Kit Exp: 9/8/2023
EZ1 Protocol: Large Volume Start time: 1159
Elution Volume: 50ul Sample Name 27 0 54 0
1 M2022-4230 Q1.1 * 28 0 55 0

2 C2022-2325 Q7.1 29 0 56 0

3 M2022-4750 Q1.6 30 0 57 0

4 M2022-4825 Q1.5 31 0 58 0

5 M2023-0257 Q1 32 0 59 0

6 M2023-0257 Q2 33 0 60 0

7 P2022-3134 Q12.1.1 34 0 61 0

8 M2023-0652 Q1.1 35 0 62 0

9 M2022-4894 Q1.5 36 0 63 0

10 M2022-4924 Q1.3 37 0 64 0

11 M2022-4843 Q125.1 38 0 65 0

12 M2022-4843 Q125.2 39 0 66 0

13 M2022-4843 Q126 40 0 67 0

14 M2022-3953 Q1 41 0 68 0

15 C2022-2295 Q2.1 42 0 69 0

16 M2022-4849 Q1.6 43 0 70 0

17 M2022-5079 Q1.6 44 0 71 0

18 P2022-3409 Q3.1 45 0 72 0

19 M2023-0427 Q1 46 0 73 0

20 M2014-2496 V2.1 47 0 74 0

21 M2022-5386 Q2 48 0 75 0

22 LVRB1 032123SAW 49 0 76 0

23 LVRB2 032123SAW 50 0 77 0

24 0 51 0 78 0 25 0 52 0 79 0 26 0 53 0 80 0
* Sample 1 dropped when loading to EZ1 tray. Lost about half of liquid, visible pellet at bottom undisturbed.

Revision: 5
Issue Date: 12/27/2022
Extraction Worksheets Page: 3 of 4 Issuing Authority: Quality Manager

22-M09903 # 34 M2022-4843

MOSCOW POLICE DEPARTMENT Page 4 of 46 JEM Extraction Worksheets DNA Extraction Form 200-BI
Batch Name: 032023SAW Work Station: DNA 3 Analyst: SAW Blood / Saliva / Tissue
Total samples: 2 Master Mix Made For: 3 ul / sample ul in Master Mix Date: 3/21/2023 SEB 081122 490 1470 56 ° C Heat Block: J2
Prok 2344719 10 30 Start Time: 0951
Exp Date: 4/28/2023 End Time: 1106 N / A Hair
Total samples: Master Mix Made For:
ul / sample ul in Master Mix Date:
SEB 0 56 ° C Heat Block:
DTT 0 Start Time:
Prok 0 End Time:
Exp. Date:
N / A Bone / Teeth
Total samples: Master Mix Made For:
ul / sample ul in Master Mix Date:
SEB 0 56 ° C Heat Block:
Prok 0 Start Time:
Exp. Date: End Time:
EZ1 Extraction Date: 3/21/2023
EZ1 Kit Lot: 172039661 EZ1:[REDACTED:US_SOCIAL_SECURITY_NUMBER]t Exp: 9/8/2023
EZ1 Protocol: Large Volume Start time: 1159
Elution Volume: 50ul Sample Name 27 0 54 0
1 M2022-5310 Q2.1 28 0 55 0

2 LVRB3 032123SAW 29 0 56 0

3 0 30 0 57 0 4 0 31 0 58 0 5 0 32 0 59 0 6 0 33 0 60 0 7 0 34 0 61 0 8 0 35 0 62 0 9 0 36 0 63 0 10 0 37 0 64 0 11 0 38 0 65 0 12 0 39 0 66 0 13 0 40 0 67 0 14 0 41 0 68 0 15 0 42 0 69 0 16 0 43 0 70 0 17 0 44 0 71 0 18 0 45 0 72 0 19 0 46 0 73 0 20 0 47 0 74 0 21 0 48 0 75 0 22 0 49 0 76 0 23 0 50 0 77 0 24 0 51 0 78 0 25 0 52 0 79 0 26 0 53 0 80 0
Revision: 5
Issue Date: 12/27/2022
Extraction Worksheets Page: 4 of 4 Issuing Authority: Quality Manager

22-M09903 # 34 M2022-4843

MOSCOW POLICE DEPARTMENT Page 5 of 46 JEM DNA Worksheets DNA Quantitation Form 206A-BI
OZ

7500 Load Sheet
Analyst: SAW
Workstation: DNA 3 Repeat Pipettes Used: No
Batch Name: 032023SAW Date: 3/21/2023 1 2 3 4 5 6 7 8 9 10 11 12
M2022- M2022- P2022- M2022- M2023- M2023- M2022- M2022- M2023- M2023-
A STD. 1A STD. 1B 4967_Q1.3F2 5118_Q1.5F2 3611_Q1.5F1 4999_Q1.5F1 1081_Q1.2F1 0257_Q1 4843_Q126 5386 Q2 1130_Q1.3 1130_Q1.4F2 P2022- M2022- M2022- M2022- M2023- M2023- M2022- LVRB1_03212 LVRB4_03212 RB_032023SA B STD. 2A STD. 2B 3611_Q1.2F2 4999_Q1.2F2 5283 Q1.2F1 4359 Q5.2F1 1081 Q1.3F1 0257 Q2 3953 Q1 3SAW 3SAW WF2
P2022- M2022- M2022- RB1 032023J RB3 032023J P2022- C2022- LVRB2_03212 M2023-
C STD. 3A STD. 3B 3611 Q1.5F2 4999_Q1.5F2 5062_Q2.2F1 EMF1 EMF1 3134 Q12.1.1 2295_Q2.1 3SAW 1130_Q1.4F1
M2022- M2022- M2022- RB2_032023J RB4_032023J M2023- M2022- M2022- RB_032023SA
D STD. 4A STD. 4B 5283_Q1.2F2 4359 Q5.2F2 5062 Q2.6F1 EMF1 EMF1 0652 Q1.1 4849 Q1.6 5310 Q2.1 WF1
M2022- RB1_032023J M2022- M2023- M2022- M2022- M2022- LVRB3_03212
E STD. 5A STD. 5B 5062_Q2.2F2 EMF2 5079_Q1.4F1 1081 Q1.2F2 4230_Q1.1 4894 Q1.5 5079_Q1.6 3SAW
M2022- RB2_032023J M2022- M2023- C2022- M2022- P2022- M2023-
F STD. 6A STD. 6B 5062_Q2.6F2 EMF2 5118 Q1.2F1 1081 Q1.3F2 2325 Q7.1 4924 Q1.3 3409 Q3.1 1130 V1.1
M2022- M2022- M2022- RB3_032023J M2022- M2022- M2023- M2023-
G STD. 7A STD. 7B 5079 Q1.4F2 4967 Q1.3F1 5118 Q1.5F1 EMF2 4750 Q1.6 4843 Q125.1 0427 Q1 1130 $ 1.2
M2022- P2022- M2022- RB4_032023J M2022- M2022- M2014- KRB_0320235
H NTCA NTCB 5118_Q1.2F2 3611_Q1.2F1 4999 Q1.2F1 EMF2 4825_Q1.5 4843_Q125.2 2496 V2.1 AW Plexor HY Kit
Lot #: 0000514519 Std. Prep. Date: 03/21/23 by SAW ΤΕ 110421
Exp. Date: 7/8/2024 7500: B
Total Samples: 86 Master Mix: 900 ul
Master Mix made for: 90 Primer / IPC Mix: 90 ul
Water: 630 ul 18 uL Master Mix / well
Revision: 9
DNA Worksheets Page: 1 of 7 Issuing Authority Issue: Date Quality: 12/27/2022 Manager

22-M09903 # 34 M2022-4843

MOSCOW POLICE DEPARTMENT Page 6 of 46 JEM DNA Worksheets
Batch Name: 032023SAW Date: 3/21/2023 Analyst: SAW Autosomal Standard Curve 0320235AW.aan-Net for Medical Diagnostic Use PCR Curves Sample IDs Standard Curves Reports FLR Curve-Autosomal 1 (group 1) CO660-Y Curve 1 group 1 Well STD Sample. 1A 20 16.9 a 80.1 Tm Conc 5.001 Yes Tm?

y-3.45x23.13 81 C1 STD STD 2A 3A 19.5 222 004 80.3 1.001 20000 Yes Yes R = 0.999 E1 D1 STD STD.. 4A SA 26.9 24.8 60.6 80.6 8.0E 4.0E - - 01 02 Yes Yes Eff = 94.95% F1 STD SA 29.4 80.5 1.6E-02 Yes A2 01 STD STD. 7A 18 176 31.7 802 80.3 3.2E 5001-03 Yes Yes 8- 82 C2 STD STD. 28 38 19.6 222 80.4 80.5 1.0E01 2.000 Yes Yes 02 STD STD.. 48 58 244 27.0 80.7 807 8.0E 4.0E - - 01 02 Yes Yes F2 02 STD STD. 68 78 29.1 31.7 80.4 80.6 1.66-02 3.2E-03 Yes Yes
R

) Threshold Cycle スー
(CE

- お 8- 2- 一番 001 01 Concentration
Revision: 9
Issue Date: 12/27/2022
DNA Worksheets Page: 2 of 7 Issuing Authority: Quality Manager

22-M09903 # 34 M2022-4843

MOSCOW POLICE DEPARTMENT Page 7 of 46 JEM DNA Worksheets
Batch Name: 032023SAW Date: 3/21/2023 Analyst: SAW Y Standard Curve 0320235AW.aan-Net for Medical Diagnostic Use.

PCR Curves Sample IDs Standard Curves | Reports FLR-Autosomal C0560- Curve 1 (group 2) Curve 1 (group 2) A1 Well STD Sample. 1A ID Ct 18.1 Tm 81.4 Conc 5.0E01 Yes Tm?

y = -3.53x + 24.36 81 C1 STO STD.. 2A ЗА 23.5 20.9 81.5 81.6 1.0E01 2.0E00 Yes Yes R = 0.995 01 STD. 4A 25.8 28.2 81.8 81.8 8.0E 4.0E - - 02 01 Yes Yes 8- Eff = 92.10% E1 F1 STD STD. SA 6A 30.6 81.8 1.6E-02 Yes 101 A2 STD STD 18,. 7A 34.1 18.6 81.7 81.4 3.2E 5.0E01-03 Yes Yes 82 C2 STD STD,. 38 28 233 20.9 81.6 81.7 1.0E01 2.0E00 Yes Yes E2 02 STD STD.. 58 48 25.4 20.2 81.9 81.9 8.0E 4.0E - - 01 02 Yes Yes F2 02 STD STD.. 68 78 30.8 32.4 81.9 81.8 1.6E 3.2E - - 02 03 Yes Yes 8- 8- 我 一 ) カー Threshold 8- Cycle Ct (0- ম- - 2 8- 2- 2- 0.01 0.1 Concentration
Revision: 9
Issue Date: 12/27/2022
DNA Worksheets Page: 3 of 7 Issuing Authority: Quality Manager

22-M09903 # 34 M2022-4843

MOSCOW POLICE DEPARTMENT Page 8 of 46 JEM DNA Worksheets DNA Quantitation Form 206B-BI 7500 Results Sheet
Batch Name: 032023SAW Analyst: SAW
Normalize to Auto / Y: Auto
Date: 3/22/2023 ΤΕ 110421
Workstation: DNA 3 Quantity Quantity ul Sample ng / µl ng / µl Auto / Y IPC for ul TE to ul to be Well Sample Name [Auto] [Y] Ratio Status Dilution be added ng / ul Final Amplified
A3 M2022-4967 Q1.3F2 N / A N / A N / A OK N / A N / A N / A N / A
B3 P2022-3611 Q1.2F2 0.072 0.0003 251.70 OK 0 0.0 0.1144 15.0
C3 P2022-3611 Q1.5F2 0.022 0.001 24.20 OK 0 0.0 0.0717 15.0

D3 M2022-5283 Q1.2F2 0.005 N / A N / A OK N / A N / A N / A N / A
E3 M2022-5062 Q2.2F2 0.291 0.200 1.50 OK 6 11.5 0.1 15.0

F3 M2022-5062 Q2.6F2 N / A N / A N / A OK N / A N / A N / A N / A
G3 M2022-5079 Q1.4F2 0.004 N / A N / A OK N / A N / A N / A N / A
H3 M2022-5118 Q1.2F2 0.221 0.029 7.60 OK 8 9.7 0.1 15.0

A4 M2022-5118 Q1.5F2 0.0004 0.0002 2.10 OK 0 0.0 0.0014 15.0
B4 M2022-4999 Q1.2F2 0.034 N / A N / A OK N / A N / A N / A N / A
C4 M2022-4999 Q1.5F2 0.018 N / A N / A OK N / A N / A N / A N / A
D4 M2022-4359 Q5.2F2 0.0003 N / A N / A OK N / A N / A N / A N / A
E4 RB1 032023JEMF2 N / A N / A N / A OK 0 0.0 0 15.0

F4 RB2 032023JEMF2 N / A N / A N / A OK N / A N / A N / A N / A
G4 M2022-4967 Q1.3F1 0.019 N / A N / A OK N / A N / A N / A N / A
H4 P2022-3611_Q1.2F1 27.900 N / A N / A OK 5 1390.0 0.1 15.0
A5 P2022-3611 Q1.5F1 9.670 0.001 11987.40 OK 5 478.5 0.1 15.0
B5 M2022-5283 Q1.2F1 4.010 N / A N / A OK N / A N / A N / A N / A
C5 M2022-5062 Q2.2F1 3.480 0.081 43.10 OK 5 169.0 0.1 15.0

D5 M2022-5062 Q2.6F1 0.001 0.0003 4.30 OK 0 0.0 0.0042 15.0

E5 M2022-5079 Q1.4F1 6.740 0.0003 22086.80 OK N / A N / A N / A N / A
F5 M2022-5118 Q1.2F1 0.799 0.0001 5752.60 OK 5 35.0 0.1 15.0
G5 M2022-5118 Q1.5F1 0.236 0.001 435.40 OK 7 9.5 0.1 15.0

H5 M2022-4999 Q1.2F1 28.900 0.0002 131974.10 OK N / A N / A N / A N / A
A6 M2022-4999 Q1.5F1 14.600 0.004 4148.50 OK N / A N / A N / A N / A
B6 M2022-4359 Q5.2F1 31.300 0.006 5553.90 OK N / A N / A N / A N / A
C6 RB1 032023JEMF1 N / A N / A N / A OK 0 0.0 0 15.0

D6 RB2 032023JEMF1 N / A N / A N / A OK N / A N / A N / A N / A
E6 M2023-1081 Q1.2F2 2.560 1.830 1.40 OK 5 123.0 0.1 15.0

F6 M2023-1081 Q1.3F2 0.006 0.004 1.60 OK 0 0.0 0.0188 15.0

G6 RB3 032023JEMF2 N / A N / A N / A OK N / A N / A N / A N / A
H6 RB4 032023JEMF2 * ^ N / A 0.0001 N / A OK 0 0.0 0.0005 15.0
A7 M2023-1081 Q1.2F1 41.500 0.278 149.00 OK 3 1242.0 0.1 15.0
B7 M2023-1081 Q1.3F1 0.062 0.001 44.10 OK 0 0.0 0.1183 15.0

C7 RB3 032023JEMF1 N / A N / A N / A OK 0 0.0 0 15.0

D7 RB4 032023JEMF1 N / A N / A N / A OK N / A N / A N / A N / A
E7 M2022-4230 Q1.1 0.218 0.144 1.50 OK 8 9.4 0.1 15.0

F7 C2022-2325 Q7.1 0.094 0.049 1.90 OK 0 0.0 0.094 15.0

G7 M2022-4750 Q1.6 0.002 0.0001 16.60 OK 0 0.0 0.0076 15.0

H7 M2022-4825 Q1.5 0.003 N / A N / A OK N / A N / A N / A N / A
A8 M2023-0257 Q1 10.300 5.990 1.70 OK 5 510.0 0.1 15.0

B8 M2023-0257 Q2 1.360 1.260 1.10 OK 5 63.0 0.1 15.0

Revision: 9
Issue Date: 12/27/2022
DNA Worksheets Page: 4 of 7 Issuing Authority: Quality Manager

22-M09903 # 34 M2022-4843

MOSCOW POLICE DEPARTMENT Page 9 of 46 JEM DNA Worksheets DNA Quantitation Form 206B-BI 7500 Results Sheet
Batch Name: 032023SAW Analyst: SAW
Normalize to Auto / Y: Auto
Date: 3/22/2023 ΤΕ 110421
Workstation: DNA 3 Quantity Quantity ul Sample ng / µl ng / µl Auto / Y IPC for ul TE to ul to be Well Sample Name [Auto] [Y] Ratio Status Dilution be added ng / ul Final Amplified
C8 P2022-3134 Q12.1.1 0.003 0.001 2.30 OK 0 0.0 0.0091 15.0

D8 M2023-0652 Q1.1 0.411 0.205 2.00 OK 5 15.6 0.1 15.0

E8 M2022-4894 Q1.5 0.016 0.005 3.10 OK 0 0.0 0.0499 15.0

F8 M2022-4924 Q1.3 0.844 N / A N / A OK 5 37.2 0.1 15.0

G8 M2022-4843 Q125.1 0.291 0.001 329.20 OK 8 15.3 0.1 15.0

H8 M2022-4843 Q125.2 0.961 0.001 659.60 OK 5 43.1 0.1 15.0

A9 M2022-4843 Q126 0.455 0.0002 2303.20 OK 5 17.8 0.1 15.0

B9 M2022-3953 Q1 0.125 0.062 2.00 OK 0 0.0 0.125 15.0

C9 C2022-2295 Q2.1 0.236 0.161 1.50 OK 8 10.9 0.1 15.0

D9 M2022-4849 Q1.6 0.002 N / A N / A OK N / A N / A N / A N / A
E9 M2022-5079 Q1.6 0.040 0.002 17.90 OK 0 0.0 0.0955 15.0

F9 P2022-3409 Q3.1 17.100 10.800 1.60 OK 5 850.0 0.1 15.0

G9 M2023-0427 Q1 0.048 0.009 5.10 OK 0 0.0 0.1152 15.0

H9 M2014-2496 V2.1 0.127 0.042 3.00 OK 0 0.0 0.127 15.0

A10 M2022-5386 Q2 0.127 0.077 1.70 OK 0 0.0 0.127 15.0

B10 LVRB1 032123SAW N / A N / A N / A OK 0 0.0 0 15.0

C10 LVRB2 032123SAW N / A N / A N / A OK N / A N / A N / A N / A
D10 M2022-5310 Q2.1 0.003 0.001 4.10 OK 0 0.0 0.008 15.0

E10 LVRB3 032123SAW N / A N / A N / A OK 0 0.0 0 15.0

A11 M2023-1130 Q1.3 1.340 N / A N / A OK N / A N / A N / A N / A
B11 LVRB4 032123SAW N / A N / A N / A OK N / A N / A N / A N / A
A12 M2023-1130 Q1.4F2 1.780 0.947 1.90 OK N / A N / A N / A N / A
B12 RB 032023SAWF2 N / A N / A N / A OK N / A N / A N / A N / A
C12 M2023-1130 Q1.4F1 1.240 0.235 5.30 OK N / A N / A N / A N / A
D12 RB 032023SAWF1 N / A N / A N / A OK N / A N / A N / A N / A
F12 M2023-1130 V1.1 0.431 N / A N / A OK N / A N / A N / A N / A
G12 M2023-1130 S1.2 1.170 0.825 1.40 OK N / A N / A N / A N / A
H12 KRB 032023SAW N / A N / A N / A OK N / A N / A N / A N / A
Note: For any concentrated samples, " ng / ul Final " reflects the new estimated concentration.

Concentrate to 15ul * Check melts flag present, no melt curve within the correct range.

Concentrate to 20ul ^ Sample normalized based on Y-value Concentrate to 25ul Concentrate to 30ul
Not Amplified Note: M2023-1130 not amplified, proficiency test
Revision: 9
Issue Date: 12/27/2022
DNA Worksheets Page: 5 of 7 Issuing Authority: Quality Manager

22-M09903 # 34 M2022-4843

MOSCOW POLICE DEPARTMENT Page 10 of 46 JEM DNA Worksheets STR Amplification Set-Up Form 206C-BI
Batch Name: 032023SAW Analyst: EMS
Plate Name: 032023SAW
Workstation: DNA 4 Repeat Pipettes Used: No
Thermal Cycler A Date: 3/22/2023 1 2 3 4 5 6 7 8 9 10 11 12
P2022- M2023- M2022-

A 3611_Q1.5F1 0257 Q1 5386_Q2

P2022- M2022- RB4_032023J M2023- M2022- LVRB1_03212

B 3611_Q1.2F2 5062_Q2.2F1 EMF2 0257_Q2 4843 Q126 3SAW

P2022- M2022- M2023- P2022- M2022- M2022-

C 3611_Q1.5F2 5062_Q2.6F1 1081_Q1.2F1 3134_Q12.1.1 3953 Q1 5310 Q2.1
M2022- M2022- M2023- M2023- C2022- LVRB3_03212

D 5062_Q2.2F2 5118_Q1.2F1 1081_Q1.3F1 0652_Q1.1 2295_Q2.1 3SAW
M2022- M2022- RB3_032023J M2022- M2022-

E 5118 Q1.2F2 5118 Q1.5F1 EMF1 4894 Q1.5 5079 Q1.6 POS_CTRL

M2022- RB1 032023J M2022- M2022- P2022-

F 5118 Q1.5F2 EMF1 4230 Q1.1 4924 Q1.3 3409 Q3.1 NEG_CTRL

RB1_032023J M2023- C2022- M2022- M2023-

G EMF2 1081_Q1.2F2 2325 Q7.1 4843 Q125.1 0427_Q1

P2022- M2023- M2022- M2022- M2014-

H 3611_Q1.2F1 1081_Q1.3F2 4750_Q1.6 4843_Q125.2 2496 V2.1

PowerPlex Fusion Kit
Lot #: 0000535695 Total Samples: 43 Master Mix Made For: 46
Exp. Date: 4/10/2025 Reagent µl / sample µl in Master Mix 5x Master Mix 5 230 TE N / A Primers 5 230
H20 0 0

Master Mix / Sample 10 DNA Template 15 Total Rxn Volume 25
Revision: 9
DNA Worksheets Page: 6 of 7 Issuing Authority Issue: Date Quality: 12/27/2022 Manager

22-M09903 # 34 M2022-4843

MOSCOW POLICE DEPARTMENT Page 11 of 46 JEM DNA Worksheets 3130 Load Sheet Form 206D-BI
ONA

Analyst: EMS
Batch Name: 032023SAW
Plate Name: 032023SAW Date: 3/22/2023 1 2 3 4 5 6 7 8 9 10 11 12
P2022- M2023- M2022-

A LADDER_A01 3611_Q1.5F1 LADDER_A03 0257_Q1 LADDER A05 5386 Q2
*
P2022- M2022- RB4_032023JE M2023- M2022- LVRB1_032123

B 3611 Q1.2F2 5062 Q2.2F1 MF2 0257_Q2 4843 Q126 SAW

P2022- M2022- M2023- P2022- M2022- M2022-

C 3611_Q1.5F2 5062 Q2.6F1 1081 Q1.2F1 3134 Q12.1.1 3953 Q1 5310 Q2.1
*
M2022- M2022- M2023- M2023- C2022- LVRB3_032123

D 5062_Q2.2F2 5118 Q1.2F1 1081_Q1.3F1 0652 Q1.1 2295 Q2.1 SAW
! *
M2022- M2022- RB3_032023JE M2022- M2022-

E 5118_Q1.2F2 5118_Q1.5F1 MF1 4894_Q1.5 5079_Q1.6 POS_CTRL

*
M2022- RB1_032023JE M2022- M2022- P2022-

F 5118_Q1.5F2 MF1 4230 Q1.1 4924 Q1.3 3409_Q3.1 NEG_CTRL

*
RB1_032023JE M2023- C2022- M2022- M2023-

G MF2 1081 Q1.2F2 2325 Q7.1 4843 Q125.1 0427_Q1 LADDER_G06

*
P2022- M2023- M2022- M2022- M2014-

H 3611_Q1.2F1 1081 Q1.3F2 4750 Q1.6 4843_Q125.2 2496_V2.1 LADDER H06
COMMENTS:

^: Reinjected for 10 seconds due to failed sizing quality, also failed, data not used. Sample reset.

*: Sample re-injected for 10 seconds due to low level data. Total Samples: 48 Master Mix made for: 52 !: Reinjected 10 seconds to match most stringent conditions.

HiDi Formamide 494 UL 3130: Jacques Internal Lane Standard 26 UL 3130 POP4 Lot #: 2211239 HiDi Formamide Lot # 2203820 Stabilizer Reagent N / A
Exp. Date: 6/10/2023 Exp. Date: 4/21/2024 Master Mix / well 10 ul Stabilizer Reagent Lot # N / A Buffer Lot #: 2203528
Exp. Date: N / A Exp. Date: 8/10/2024
Revision: 9
DNA Worksheets Page: 7 of 7 Issuing Authority Issue: Date Quality: 12/27/2022 Manager

22-M09903 # 34 M2022-4843

MOSCOW POLICE DEPARTMENT Page 12 of 46 JEM DNA Worksheets 3130 Load Sheet Form 206D-BI
ONA

Analyst: KNB
Batch Name: 032023SAW
Plate Name: 032023SAW RESET Date: 3/24/2023 1 2 3 4 5 6 7 8 9 10 11 12
A LADDER A01

M2022-

B 5118 Q1.5F2

C LADDER_C01

D LADDER D01

E

F

G

H

COMMENTS:

Total Samples: 4 Master Mix made for: 5 HiDi Formamide 47.5 uL 3130: Jacques Internal Lane Standard 2.5 uL 3130 POP4 Lot #: 2211239 HiDi Formamide Lot # 2203820 Stabilizer Reagent N / A
Exp. Date: 6/10/2023 Exp. Date: 4/21/2024 Master Mix / well 10 UL Stabilizer Reagent Lot # N / A Buffer Lot #. 2203528
Exp. Date: N / A Exp. Date: 8/10/2024
Revision: 9
DNA Worksheets Page: 1 of 1 Issuing Authority Issue: Date Quality: 12/27/2022 Manager

22-M09903 # 34 M2022-4843

MOSCOW POLICE DEPARTMENT Page 13 of 46 JEM
██████████████████ 032023SAW Imported 1 GeneMapper ID v3.2.1 Sample Name ████████████████████████████████████████ ████████████████████████████████████████ ████████████████████████████████████████ ████████████████████████████████████████ ████████████████████████████████████████ ████████████████████████████████████████ ██████████████████