Document
22-M09903 # 13 M2022-4843 JEM MOSCOW POLICE DEPARTMENT Page 1 of 349 Extraction Worksheets Differential DNA Extraction Form 202B-BI Batch Name: 112922ΚΝΗ Work Station: DNA 2 Analyst: ΚΝΗ Differential Extraction (F1) Date: 11/29/2022 4 ° C Start Time: 11/29/22 1457 150 µl PBS 070722 End Time: 11/30/220830 Date: 11/30/2022 Total samples: 12 Master Mix Made For: 13 ul / sample ul in Master Mix 56 ° C Heat Block: G1 SEB 090121 190 2470 Start Time: 0936 Prok 2344719 10 130 End Time: 1023 Exp. Date: 4/28/2023 Differential Extraction (F2) Manual 500 pl PBS 070722 x3 500 ul dH2O x1 Sperm Lysis Total samples: 12 Master Mix Made For: 13 ul / sample ul in Master Mix SEB 090121 180 2340 56 ° C Heat Block: G1 DTT 021722 10 130 Start Time: 1152 Prok 2344719 10 130 End Time: 1402 Exp. Date: 4/28/2023 EZ1 Extraction EZ1 Kit Lot: 172033389 [REDACTED:US_SOCIAL_SECURITY_NUMBER]30/2022 EZ1 Kit Exp: 4/21/2023 EZ1: F2: G EZ1 Protocol: Trace F1: E Elution Volume: 50 uL Start time: F2: 1425 F1: 1434 Sample Name Sample Name 1 M2022-2722 Q1.3 13 2 M2022-2787 Q1.2 14 3 M2022-2787 Q1.5 15 4 M2022-2797 Q1.2 16 5 M2022-2797 Q1.6 * 17 6 M2022-2850 Q1.2 18 7 M2022-2850 Q1.3 19 8 M2022-2931 Q1.2 20 9 M2022-2931 Q1.4 21 10 M2022-2722 Q2.2 22 11 RB1_112922KNH 23 12 RB2 112922ΚΝΗ 24 * F1 of sample re-run on EZ1 due to no elution volume. Revision: 4 Issue Date: 05/31/2022 Extraction Worksheets Page: 1 of 4 Issuing Authority: Quality Manager 22-M09903 # 13 M2022-4843 JEM MOSCOW POLICE DEPARTMENT Page 2 of 349 Extraction Worksheets Differential DNA Extraction Form 202B-BI Batch Name: 112922ΚΝΗ Work Station: DNA 3 Analyst: EMS Differential Extraction (F1) Date: 11/29/2022 4 ° C Start Time: 1425 150 µl PBS 070722 End Time: 11/30/2022 0712 Date: 11/30/2022 Total samples: 4 Master Mix Made For: N / A ul / sample ul in Master Mix 56 ° C Heat Block: J1 SEB 081122 190 N / A Start Time: 0750 Prok 2344719 10 N / A End Time: 0820 Exp. Date: 4/28/2023 Differential Extraction (F2) Manual 500 pl PBS 070722 X3 500 ul dH2O Sperm Lysis Total samples: 4 Master Mix Made For: N / A ul / sample ul in Master Mix SEB 081122 180 N / A 56 ° C Heat Block: J1 DTT 021722 10 N / A Start Time: 0940 Prok 2344719 10 N / A End Time: 1035 Exp. Date: 4/28/2023 EZ1 Extraction EZ1 Kit Lot: 172033389 Date: 11/3[REDACTED:US_SOCIAL_SECURITY_NUMBER]1 Kit Exp: 4/21/2023 EZ1: E EZ1 Protocol: Trace Elution Volume: 50 ul Start time: 1052 Sample Name Sample Name 1 M2021-3876 Q1.2RE 13 2 M2022-4574 Q3.2 14 3 RB1 112922EMS 15 4 RB2 112922EMS 16 5 17 6 18 7 19 8 20 9 21 10 22 11 23 12 24 Revision: 4 Issue Date: 05/31/2022 Extraction Worksheets Page: 2 of 4 Issuing Authority: Quality Manager 22-M09903 # 13 M2022-4843 JEM MOSCOW POLICE DEPARTMENT Page 3 of 349 Extraction Worksheets DNA Extraction Form 200-BI Batch Name: 112922ΚΝΗ Work Station: DNA 3 Analyst: EMS Blood / Saliva / Tissue Total samples: 13 Master Mix Made For: 15 ul / sample ul in Master Mix Date: 11/29/2022 SEB 081122 490 7350 56 ° C Heat Block: J1 Prok 2344719 10 150 Start Time: 11/29/2022 1450 Exp Date: 4/28/2023 End Time: 11/30/2022 0720 N / A Hair Total samples: Master Mix Made For: ul / sample ul in Master Mix Date: SEB 0 56 ° C Heat Block: DTT 0 Start Time: Prok 0 End Time: Exp. Date: N / A Bone / Teeth Total samples: Master Mix Made For: ul / sample ul in Master Mix Date: SEB 0 56 ° C Heat Block: Prok 0 Start Time: Exp. Date: End Time: EZ1 Extraction Date: 11/30/2022 EZ1 Kit Lot: 172033389 EZ1: E EZ1 Kit E[REDACTED:US_SOCIAL_SECURITY_NUMBER]2023 EZ1 Protocol: Large Volume Start time: 0950 Elution Volume: 50 ul Sample Name 27 0 54 0 1 M2022-4582 Q7.1.1 28 0 55 0 2 M2022-4582 Q7.1.2 29 0 56 0 3 M2022-4582 Q7.1.3 30 0 57 0 4 M2022-4582 Q7.1.4 31 0 58 0 5 M2022-4582 Q3 ^ 32 0 59 0 6 M2022-4582 Q6.1.1 33 0 60 0 7 M2022-4582 Q6.2.1 34 0 61 0 8 M2022-4582 Q5.1 35 0 62 0 9 M2022-4656 Q1 36 0 63 0 10 M2022-4656 Q2 37 0 64 0 11 M2022-4843 Q24 38 0 65 0 12 LVRB1 112922EMS 39 0 66 0 13 LVRB2 112922EMS 40 0 67 0 14 0 41 0 68 0 15 0 42 0 69 0 16 0 43 0 70 0 17 0 44 0 71 0 18 0 45 0 72 0 19 0 46 0 73 0 20 0 47 0 74 0 21 0 48 0 75 0 22 0 49 0 76 0 23 0 50 0 77 0 24 0 51 0 78 0 25 0 52 0 79 0 26 0 53 0 80 0 ^ No elution volume and sample tube discarded, sample to be recut. Revision: 4 Issue Date: 05/31/2022 Extraction Worksheets Page: 3 of 4 Issuing Authority: Quality Manager 22-M09903 # 13 M2022-4843 JEM MOSCOW POLICE DEPARTMENT Page 4 of 349 Extraction Worksheets DNA Extraction Form 200-BI Batch Name: 112922ΚΝΗ Work Station: DNA 3 Analyst: EMS Blood / Saliva / Tissue Total samples: 8 Master Mix Made For: 9 ul / sample ul in Master Mix Date: 11/29/2022 SEB 081122 490 4410 56 ° C Heat Block: J2 Prok 2344719 10 90 Start Time: 11/29/2022 1455 Exp Date: 4/28/2023 End Time: 11/30/2022 0720 N / A Hair Total samples: Master Mix Made For: ul / sample ul in Master Mix Date: SEB 0 56 ° C Heat Block: DTT 0 Start Time: Prok 0 End Time: Exp. Date: N / A Bone / Teeth Total samples: Master Mix Made For: ul / sample ul in Master Mix Date: SEB 0 56 ° C Heat Block: Prok 0 Start Time: Exp. Date: End Time: EZ1 Extraction Date: 11/30/2022 EZ1 Kit Lot: 172033389 EZ1: G EZ1 Kit Exp: 4/[REDACTED:US_SOCIAL_SECURITY_NUMBER]Z1 Protocol: Large Volume Start time: 0955 Elution Volume: 50 ul Sample Name 27 0 54 0 1 M2022-4843 Q23 28 0 55 0 2 M2022-4843 Q29.1 29 0 56 0 3 M2022-4843 Q29.2 30 0 57 0 4 M2022-4843 Q29.3 31 0 58 0 5 M2022-4843 Q30 32 0 59 0 6 M2022-4843 Q31.1 33 0 60 0 7 LVRB3 112922EMS 34 0 61 0 8 LVRB4 112922EMS 35 0 62 0 9 0 36 0 63 0 10 0 37 0 64 0 11 0 38 0 65 0 12 0 39 0 66 0 13 0 40 0 67 0 14 0 41 0 68 0 15 0 42 0 69 0 16 0 43 0 70 0 17 0 44 0 71 0 18 0 45 0 72 0 19 0 46 0 73 0 20 0 47 0 74 0 21 0 48 0 75 0 22 0 49 0 76 0 23 0 50 0 77 0 24 0 51 0 78 0 25 0 52 0 79 0 26 0 53 0 80 0 Revision: 4 Issue Date: 05/31/2022 Extraction Worksheets Page: 4 of 4 Issuing Authority: Quality Manager 22 MOSCOW-M09903 POLICE # 13 DEPARTMENT M2022-4843 Page 5 of 349 JEM DNA Worksheets DNA Quantitation Form 206A-BI ONA 7500 Load Sheet Analyst: KNH Workstation: DNA 2 Repeat Pipettes Used: Yes Batch Name: 112922ΚΝΗ Date: 12/1/2022 1 2 3 4 5 6 7 8 9 10 11 12 M2021- M2022- M2022- M2022- 3876_Q1.2RE M2022- M2022- M2022- A STD. 1A STD. 1B 2722_Q1.3F2 2931_Q1.4F2 2797_Q1.6F1 F2 4582_Q7.1.1 4656 Q2 4843_Q30 M2022- M2022- M2022- M2022- M2022- M2022- M2022- B STD. 2A STD. 2B 2787_Q1.2F2 2722_Q2.2F2 2850_Q1.2F1 4574_Q3.2F2 4582_Q7.1.2 4843 Q24 4843 Q31.1 M2022- RB1_112922K M2022- RB1_112922E M2022- LVRB1_11292 LVRB3_11292 C STD. 3A STD. 3B 2787_Q1.5F2 NHF2 2850_Q1.3F1 MSF2 4582_Q7.1.3 2EMS 2EMS M2022- RB2_112922K M2022- RB2_112922E M2022- LVRB2_11292 LVRB4_11292 D STD. 4A STD. 4B 2797_Q1.2F2 NHF2 2931 Q1.2F1 MSF2 4582_Q7.1.4 2EMS 2EMS M2021- M2022- M2022- M2022- 3876_Q1.2RE M2022- M2022- E STD. 5A STD. 5B 2797_Q1.6F2 2722_Q1.3F1 2931_Q1.4F1 F1 4582_Q6.1.1 4843_Q23 M2022- M2022- M2022- M2022- M2022- M2022- F STD. 6A STD. 6B 2850_Q1.2F2 2787_Q1.2F1 2722_Q2.2F1 4574_Q3.2F1 4582_Q6.2.1 4843 Q29.1 M2022- M2022- RB1_112922K RB1_112922E M2022- M2022- G STD. 7A STD. 7B 2850_Q1.3F2 2787_Q1.5F1 NHF1 MSF1 4582_Q5.1 4843_Q29.2 M2022- M2022- RB2_112922K RB2_112922E M2022- M2022- H NTCA NTCB 2931_Q1.2F2 2797_Q1.2F1 NHF1 MSF1 4656 Q1 4843 Q29.3 Plexor HY Kit Lot #: 0000514519 Std. Prep. Date: 12/1/22 by KNΝΗ ΤΕ 021221 Exp. Date: 7/8/2024 7500: A Total Samples: 68 Master Mix: 750 ul Master Mix made for: 75 Primer / IPC Mix: 75 ul Water: 525 ul 18 uL Master Mix / well Issue Date: 06/01/2022 Revision: 8 DNA Worksheets Page: 1 of 7 Issuing Authority: Quality Manager 22-M09903 # 13 M2022-4843 JEM MOSCOW POLICE DEPARTMENT Page 6 of 349 DNA Worksheets Batch Name: 112922KNH Date: 12/1/2022 Analyst: ΚΝΗ Autosomal Standard Curve Plexor (R) Analysis Software-v1.5.4.18 X File Edit View Tools Forensics Windour Help H 112922KNH.zan-Net for Medical Diagnostic Use. -Y PCR Curves Sample IDs Standard Curves Reports Curve FLR-Autosomal 1 (group 1) CC560 | - Y Curve 1 (group 1) Well STD Sample. 1A ID 17.1 Ct Tm 80.1 Conc 5.0E01 Yes Tn? y = -3.49x + 23.09 B1 C1 STD STD.. 2A 3A 22.1 19.4 80.4 80.3 2.0E00 1.0E01 Yes Yes Eff R² 93.36 = 0.997% E1 D1 STD STD.. 4A 5A 24.5 26.9 00.6 80.6 8.0E 4.0E - - 02 01 Yes Yes G1 STD STD., 6A TA 29.3 32.1 80.5 80.3 1.6E 3.26-03-02 Yes Yos 30- AZ B2 BCDEFG STD STD.. 2B 1B 17.2 19.6 80.2 80.4 1.0E01 5.0E01 Yes Yes D2 STD STD. 3B. 4B 22.2 24.3 80.5 60.6 4.0E 2.0E00-01 Yes Yes 8- STD STD.. 6B 5B 26.9 20.0 80.8 80.6 8.0E 1.6E - - 02 02 Yes Yoc STD.? B 31.1 80.4 3.26-03 Yes 20- 22 8- Threshold 28- Cyole Ct ( 8- 2- 22 20- 2- 17 0.01 0.1 Concentration Revision: 8 Issue Date: 06/01/2022 DNA Worksheets Page: 2 of 7 Issuing Authority: Quality Manager 22-M09903 # 13 M2022-4843 JEM MOSCOW POLICE DEPARTMENT Page 7 of 349 DNA Worksheets Batch Name: 112922ΚΝΗ Date: 12/1/2022 Analyst: KNH Y Standard Curve Plexor (R) Analysis Software-v1.5.4.18 X File Edit Vieve Tools Forensics Window Help $ 1800 112921KNH.aan-Not fer Medical Diagnostic Use. PCR. Curves Sample IDs Standard Curves Reports FLR-Autosomal CO560-Y Curve 1 (group 2) Curve 1 (group 2) A1 Well STD Samp ample. 1A ID Ct 18.9 91.3 Tm Conc 6.0E01 Tm Yes? 33- y = -3.42x 2x + 24.63 B1 C1 STD STD.. ZA 3A 21.3 23.7 81.5 81.6 1.0E01 2.0E00 Yes Yes R2 = 0.998 D1 STD. 4A 26.1 20.5 01.0 81.8 8.0E 4.0E - - 01 02 Yes Yes Eff96 96.12% E1 F1 STD STD.. 5A 6A 31.2 81.8 1.6E-02 Yes 3- G1 A2 STD STD.. 7A 18 33.5 18.8 81.6 81.4 3.2E 5.0E01-03 Yes Yes B2 C2 STD STD. 28. 39 21.0 23.5 81.6 01.8 1.0E01 2.0E00 Yes Yes D2 STD STD.. 4B 58 26.8 28.1 81.8 82.0 4.0E 8.0E - - 02 01 Yes Yes 30 F2 STD STD.. 68 78 30.7 32.6 81.9 81.8 1.6E 3.2E - - 02 03 Yes Yes 8- 2- 8- ) Threshold 2- Cycle 8- Ct (20- - -8 -B 221 8- 하 0.01 0.1 10 Concentration Revision: 8 Issue Date: 06/01/2022 DNA Worksheets Page: 3 of 7 Issuing Authority: Quality Manager 22-M09903 # 13 M2022-4843 JEM MOSCOW POLICE DEPARTMENT Page 8 of 349 DNA Worksheets DNA Quantitation Form 206B-BI DNA 7500 Results Sheet Batch Name: 112922ΚΝΗ Analyst: KNH Normalize to Auto / Y: Auto Date: 12/2/2022 ΤΕ 021221 Workstation: DNA 2 Quantity Quantity ul Sample ng / µl ng / µl Auto / Y IPC for ul TE to ul to be Well Sample Name [Auto] [Y] Ratio Status Dilution be added ng / ul Final Amplified A3 M2022-2722 Q1.3F2 0.079 0.013 6.00 OK 0 0.0 0.108 15.0 B3 M2022-2787_Q1.2F2 0.042 N / A N / A OK N / A N / A N / A N / A C3 M2022-2787 Q1.5F2 0.043 N / A N / A OK N / A N / A N / A N / A D3 M2022-2797 Q1.2F2 0.008 0.0002 33.00 OK 0 0.0 0.0256 15.0 E3 M2022-2797 Q1.6F2 ^ N / A 0.001 N / A OK 0 0.0 0.0032 15.0 F3 M2022-2850 Q1.2F2 0.045 N / A N / A OK N / A N / A N / A N / A G3 M2022-2850 Q1.3F2 0.0002 N / A N / A OK N / A N / A N / A N / A H3 M2022-2931 Q1.2F2 0.057 N / A N / A OK N / A N / A N / A N / A A4 M2022-2931_Q1.4F2 0.009 0.0002 41.90 OK 0 0.0 0.0288 15.0 B4 M2022-2722 Q2.2F2 0.024 0.013 1.90 OK 0 0.0 0.0768 15.0 C4 RB1_112922KNHF2 N / A N / A N / A OK 0 0.0 0 15.0 D4 RB2 112922KNHF2 N / A N / A N / A OK N / A N / A N / A N / A E4 M2022-2722 Q1.3F1 46.200 0.0095 4861.10 OK 3 1383.0 0.1 15.0 F4 M2022-2787 Q1.2F1 48.900 0.0002 313336.40 OK N / A N / A N / A N / A G4 M2022-2787 Q1.5F1 14.300 N / A N / A OK N / A N / A N / A N / A H4 M2022-2797 Q1.2F1 26.900 0.001 37667.90 OK 5 1340.0 0.1 15.0 A5 M2022-2797 Q1.6F1 0.055 N / A N / A OK 0 0.0 0.1056 15.0 B5 M2022-2850_Q1.2F1 18.800 N / A N / A OK N / A N / A N / A N / A C5 M2022-2850 Q1.3F1 0.211 0.0004 473.10 OK N / A N / A N / A N / A D5 M2022-2931 Q1.2F1 18.700 0.004 5292.00 OK N / A N / A N / A N / A E5 M2022-2931_Q1.4F1 8.480 0.003 2487.70 OK 5 419.0 0.1 15.0 F5 M2022-2722 Q2.2F1 0.550 0.280 2.00 OK 5 22.5 0.1 15.0 G5 RB1 112922KNHF1 N / A N / A N / A OK 0 0.0 0 15.0 H5 RB2 112922KNHF1 N / A N / A N / A OK N / A N / A N / A N / A A6 M2021-3876 Q1.2REF2 0.097 0.027 3.60 OK 0 0.0 0.097 15.0 B6 M2022-4574 Q3.2F2 0.003 0.004 0.70 OK 0 0.0 0.0096 15.0 C6 RB1_112922EMSF2 N / A N / A N / A OK 0 0.0 0 15.0 D6 RB2 112922EMSF2 N / A N / A N / A OK N / A N / A N / A N / A E6 M2021-3876 Q1.2REF1 5.140 0.006 878.90 OK 5 252.0 0.1 15.0 F6 M2022-4574 Q3.2F1 5.230 N / A N / A OK 5 256.5 0.1 15.0 G6 RB1 112922EMSF1 N / A N / A N / A OK 0 0.0 0 15.0 H6 RB2_112922EMSF1 N / A N / A N / A OK N / A N / A N / A N / A A7 M2022-4582_Q7.1.1 0.277 0.138 2.00 OK 10 17.7 0.1 15.0 B7 M2022-4582 Q7.1.2 0.030 0.001 53.50 OK 0 0.0 0.096 15.0 C7 M2022-4582 Q7.1.3 0.011 0.004 2.60 OK 0 0.0 0.0352 15.0 D7 M2022-4582 Q7.1.4 N / A N / A N / A OK N / A N / A N / A N / A E7 M2022-4582 Q6.1.1 2.060 0.322 6.40 OK 5 98.0 0.1 15.0 F7 M2022-4582 Q6.2.1 0.191 0.002 98.90 OK 10 9.1 0.1 15.0 G7 M2022-4582 Q5.1 3.480 2.270 1.50 OK 5 169.0 0.1 15.0 H7 M2022-4656 Q1 18.700 17.800 1.10 OK 5 930.0 0.1 15.0 A8 M2022-4656_Q2 8.190 5.950 1.40 OK 5 404.5 0.1 15.0 B8 M2022-4843 Q24 1.150 0.005 239.70 OK 5 52.5 0.1 15.0 Revision: 8 Issue Date: 06/01/2022 DNA Worksheets Page: 4 of 7 Issuing Authority: Quality Manager 22-M09903 # 13 M2022-4843 JEM MOSCOW POLICE DEPARTMENT Page 9 of 349 DNA Worksheets DNA Quantitation Form 206B-BI DNA 7500 Results Sheet Batch Name: 112922ΚΝΗ Analyst: KNH Normalize to Auto / Y: Auto Date: 12/2/2022 ΤΕ 021221 Workstation: DNA 2 Quantity Quantity ul Sample ng / µl ng / µl Auto / Y IPC for ul TE to ul to be Well Sample Name [Auto] [Y] Ratio Status Dilution be added ng / ul Final Amplified C8 LVRB1_112922EMS N / A N / A N / A OK 0 0.0 0 15.0 D8 LVRB2 112922EMS N / A N / A N / A OK N / A N / A N / A N / A E8 M2022-4843 Q23 0.100 N / A N / A OK 0 0.0 0.1 15.0 F8 M2022-4843 Q29.1 0.0004 N / A N / A OK 0 0.0 0.00128 15.0 G8 M2022-4843 Q29.2 N / A N / A N / A OK N / A N / A N / A N / A H8 M2022-4843 Q29.3 0.0001 N / A N / A OK 0 0.0 0.00032 15.0 A9 M2022-4843 Q30 0.033 0.014 2.40 OK 0 0.0 0.1056 15.0 B9 M2022-4843 Q31.1 0.679 0.495 1.40 OK 5 29.0 0.1 15.0 C9 LVRB3 112922EMS N / A N / A N / A OK 0 0.0 0 15.0 D9 LVRB4 112922EMS N / A N / A N / A OK N / A N / A N / A N / A Note: For any concentrated samples, " ng / ul Final " reflects the new estimated concentration. Not Amplified ^ Sample normalized to the Y value Concentrated to 15 ul Concentrated to 25 ul Concentrated to 35 uL Revision: 8 Issue Date: 06/01/2022 DNA Worksheets Page: 5 of 7 Issuing Authority: Quality Manager 22-M09903 # 13 M2022-4843 JEM MOSCOW POLICE DEPARTMENT Page 10 of 349 DNA Worksheets STR Amplification Set-Up Form 206C-BI Batch Name: 112922ΚΝΗ Analyst: EMS Plate Name: 112922ΚΝΗ Workstation: DNA 4 Repeat Pipettes Used: No Thermal Cycler A Date: 12/5/2022 1 2 3 4 5 6 7 8 9 10 11 12 M2022- M2022- A 2797_Q1.2F1 4582_Q6.2.1 M2021- M2022- M2022- 3876_Q1.2RE M2022- M2022- B 2722_Q1.3F2 2797_Q1.6F1 F1 4582_Q5.1 4843 Q29.3 M2022- M2022- M2022- M2022- M2022- C 2797_Q1.2F2 2931_Q1.4F1 4574_Q3.2F1 4656_Q1 4843_Q30 M2022- M2022- RB1_112922E M2022- M2022- D 2797_Q1.6F2 2722_Q2.2F1 MSF1 4656_Q2 4843_Q31.1 M2022- RB1_112922K M2022- M2022- LVRB3_11292 E 2931_Q1.4F2 NHF1 4582_Q7.1.1 4843_Q24 2EMS M2021- M2022- 3876_Q1.2RE M2022- LVRB1_11292 F 2722 Q2.2F2 F2 4582_Q7.1.2 2EMS POS_CTRL RB1_112922K M2022- M2022- M2022- G NHF2 4574_Q3.2F2 4582_Q7.1.3 4843 Q23 NEG_CTRL M2022- RB1_112922E M2022- M2022- H 2722_Q1.3F1 MSF2 4582_Q6.1.1 4843 Q29.1 PowerPlex Fusion Kit Lot #: 0000527711 Total Samples: 36 Master Mix Made For: 40 Exp. Date: 2/7/2025 Reagent µl / sample µl in Master Mix 5x Master Mix 5 200 TE N / A Primers 5 200 H20 0 0 Master Mix / Sample 10 DNA Template 15 Total Rxn Volume 25 Issue Date: 06/01/2022 Revision: 8 DNA Worksheets Page: 6 of 7 Issuing Authority: Quality Manager 22 MOSCOW-M09903 POLICE # 13 DEPARTMENT M2022-4843 Page 11 of 349 JEM DNA Worksheets 3130 Load Sheet Form 206D-BI OZC Analyst: EMS Batch Name: 112922ΚΝΗ Plate Name: 112922ΚΝΗ Date: 12/5/2022 1 2 3 4 5 6 7 8 9 10 11 12 M2022- M2022- A LADDER_A08 2797_Q1.2F1 LADDER_A10 4582_Q6.2.1 LADDER_A12 M2021- M2022- M2022- 3876_Q1.2REF M2022- M2022- B 2722_Q1.3F2 2797_Q1.6F1 1 4582_Q5.1 4843 Q29.3 A M2022- M2022- M2022- M2022- M2022- C 2797_Q1.2F2 2931_Q1.4F1 4574_Q3.2F1 4656_Q1 4843_Q30 A M2022- M2022- RB1_112922E M2022- M2022- D 2797_Q1.6F2 2722_Q2.2F1 MSF1 4656 Q2 4843_Q31.1 M2022- RB1_112922K M2022- M2022- LVRB3_112922 E 2931 Q1.4F2 NHF1 4582 Q7.1.1 4843_Q24 EMS M2021- M2022- 3876_Q1.2REF M2022- LVRB1_112922 F 2722_Q2.2F2 2 4582 Q7.1.2 EMS POS_CTRL % RB1_112922K M2022- M2022- M2022- G NHF2 4574_Q3.2F2 4582_Q7.1.3 4843_Q23 NEG_CTRL A M2022- RB1_112922E M2022- M2022- H 2722_Q1.3F1 MSF2 4582_Q6.1.1 4843_Q29.1 LADDER_H12 COMMENTS: *: Reinjected to confirm OL allele ^: Reinjected for 10 seconds due to low level data; RB to match most stringent conditions Total Samples: 40 Master Mix made for: 44 %: Reinjected due to ILS failure Original Ladder_A12 data sizing used in quality STŔmix adjusted project manually for M2022-4582_Q7.1.3 in STRmix import file HiDi Formamide 418 uL 3130: Jacques Internal Lane Standard 22 uL 3130 POP4 Lot #: 2205233 HiDi Formamide Lot #: 2203820 Stabilizer Reagent N / A Exp. Date: 2/19/2023 Exp. Date: 4/21/2024 Master Mix / well 10 uL Stabilizer Reagent Lot #: N / A Buffer Lot #: 2203528 Exp. Date: N / A Exp. Date: 8/10/2024 Issue Date: 06/01/2022 Revision: 8 DNA Worksheets Page: 7 of 7 Issuing Authority: Quality Manager 22-M09903 # 13 M2022-4843 MOSCOW POLICE DEPARTMENT Page 12 of 349 JEM Extraction Worksheets DNA Extraction Form 200-BI Batch Name: 120722ΚΝΗ Work Station: DNA 2 Analyst: ΚΝΗ Blood / Saliva / Tissue Total samples: 12 Master Mix Made For: 13 ul / sample ul in Master Mix Date: 12/7/2022 SEB 090121 490 6370 56 ° C Heat Block: G1 Prok 2344719 10 130 Start Time: 12/7/22 1626 Exp Date: 4/28/2023 End Time: 12/8/22 0758 N / A Hair Total samples: Master Mix Made For: ul / sample ul in Master Mix Date: SEB 0 56 ° C Heat Block: DTT 0 Start Time: Prok 0 End Time: Exp. Date: N / A Bone / Teeth Total samples: Master Mix Made For: ul / sample ul in Master Mix Date: SEB 0 56 ° C Heat Block: Prok 0 Start Time: Exp. Date: End Time: EZ1 Extraction Date: 12/8/2022 EZ1 Kit Lot: 172033389 EZ1: G EZ1 Kit Exp: 4/21/2023 EZ1 Protocol: Large Volume Start time: 0844 El[REDACTED:US_SOCIAL_SECURITY_NUMBER]ume: 50 uL Sample Name 27 0 54 0 1 M2022-4843 Q37.1 28 0 55 0 2 M2022-4843 Q37.2 29 0 56 0 3 M2022-4843 Q38 * 30 0 57 0 4 M2022-4843 Q39 31 0 58 0 5 M2022-4843 Q40.1 32 0 59 0 6 M2022-4843 Q40.2 33 0 60 0 7 M2022-4843 Q40.3 34 0 61 0 8 M2022-4843 Q40.4 35 0 62 0 9 M2022-4843 Q1.5 36 0 63 0 10 M2021-4407 Q5.1 37 0 64 0 11 LVRB1 120722ΚΝΗ 38 0 65 0 12 LVRB2 120722ΚΝΗ 39 0 66 0 13 0 40 0 67 0 14 0 41 0 68 0 15 0 42 0 69 0 16 0 43 0 70 0 17 0 44 0 71 0 18 0 45 0 72 0 19 0 46 0 73 0 20 0 47 0 74 0 21 0 48 0 75 0 22 0 49 0 76 0 23 0 50 0 77 0 24 0 51 0 78 0 25 0 52 0 79 0 26 0 53 0 80 0 * EZ1 elution of sample is cloudy Revision: 4 Issue Date: 05/31/2022 Extraction Worksheets Page: 1 of 1 Issuing Authority: Quality Manager 22-M09903 # 13 M2022-4843 MOSCOW POLICE DEPARTMENT Page 13 of 349 JEM DNA Worksheets DNA Quantitation Form 206A-BI OZA 7500 Load Sheet Analyst: KNH Workstation: DNA 2 Repeat Pipettes Used: Yes Batch Name: 120722ΚΝΗ Date: 12/8/2022 1 2 3 4 5 6 7 8 9 10 11 12 M2022- M2022- A STD. 1A STD. 1B 4843 Q37.1 4843_Q1.5 M2022- M2021- B STD. 2A STD. 2B 4843_Q37.2 4407_Q5.1 M2022- LVRB1_12072 C STD. 3A STD. 3B 4843 Q38 2KNH M2022- LVRB2_12072 D STD. 4A STD. 4B 4843 Q39 2KNH M2022- E STD. 5A STD. 5B 4843 Q40.1 M2022- F STD. 6A STD. 6B 4843 Q40.2 M2022- G STD. 7A STD. 7B 4843 Q40.3 M2022- H NTCA NTCB 4843 Q40.4 Plexor HY Kit Lot #: 0000514519 Std. Prep. Date: 12/8/22 by JEM ΤΕ 110421 Exp. Date: 7/8/2024 7500: B Total Samples: 28 Master Mix: 320 ul Master Mix made for: 32 Primer / IPC Mix: 32 ul Water: 224 ul 18 uL Master Mix / well Revision: 8 DNA Worksheets Page: 1 of 6 Issuing Authority Issue Date: Quality: 06/01/2022 Manager 22-M09903 # 13 M2022-4843 MOSCOW POLICE DEPARTMENT Page 14 of 349 JEM DNA Worksheets Batch Name: 120722ΚΝΗ Date: 12/8/2022 Analyst: ΚΝΗ Autosomal Standard Curve Plexor (R) Analysis Software-v1.5.4.18 X File Edit